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il-12/il-23 p40 (total) mouse uncoated elisa kit  (Thermo Fisher)


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    Thermo Fisher il-12/il-23 p40 (total) mouse uncoated elisa kit
    Il 12/Il 23 P40 (Total) Mouse Uncoated Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il-12/il-23 p40 (total) mouse uncoated elisa kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    il-12/il-23 p40 (total) mouse uncoated elisa kit - by Bioz Stars, 2026-03
    90/100 stars

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    FPF treatment inhibited proinflammatory protein expression and AP-1 and STAT3 activation in the CIA mouse model. C57BL/6 mice were injected intradermally with CII at the base of the tail on day 0 and then challenged with 50 μ g CII subplantar at both hind limbs on day 18. On day 46, the paws and the spleen were dissected and total proteins were extracted ( n = 3-4 each group). Expressions of phosphorylated (p-) Jun D+c-Jun (a) and p-STAT3 (b) in paws and MCP-1 in the spleen (c) were determined using western blot and quantified by densitometric analyses. All data are mean ± standard error of the mean (SEM). ∗ P < 0.05 and ∗∗ P < 0.01 vs. the vehicle-treated control as determined using Student's t -test.

    Journal: Mediators of Inflammation

    Article Title: Flemingia philippinensis Flavonoids Relieve Bone Erosion and Inflammatory Mediators in CIA Mice by Downregulating NF- κ B and MAPK Pathways

    doi: 10.1155/2019/5790291

    Figure Lengend Snippet: FPF treatment inhibited proinflammatory protein expression and AP-1 and STAT3 activation in the CIA mouse model. C57BL/6 mice were injected intradermally with CII at the base of the tail on day 0 and then challenged with 50 μ g CII subplantar at both hind limbs on day 18. On day 46, the paws and the spleen were dissected and total proteins were extracted ( n = 3-4 each group). Expressions of phosphorylated (p-) Jun D+c-Jun (a) and p-STAT3 (b) in paws and MCP-1 in the spleen (c) were determined using western blot and quantified by densitometric analyses. All data are mean ± standard error of the mean (SEM). ∗ P < 0.05 and ∗∗ P < 0.01 vs. the vehicle-treated control as determined using Student's t -test.

    Article Snippet: Antibodies to the following proteins were purchased from Santa Cruz Biotechnology, Santa Cruz, CA: goat anti-mouse IgG2a-HRP (sc-2061), MMP-9 (sc-393859), cathepsin K (sc-48353), MCP-1 (sc-52701), phosphorylated STAT3 (sc-8059), phosphorylated I- κ B α (sc-8404), and phosphorylated NF- κ Bp65 (sc-101753); antibodies anti-Mouse IgG1-HRP, phosphorylated JNK1+JNK2+JNK3 (ab124956), phosphorylated p38 MAPK (ab178867), and phosphorylated Jun D+c-Jun (ab208035) were purchased from Abcam; phosphorylated ERK1/2 antibody (4370) was from Cell Signaling Technology, GAPDH antibody was from PeproTech Biotechnology, and ELISA kits for TNF- α , IL-10, IL-12/IL-23 p40 (total), and MCP-1 were from eBioscience.

    Techniques: Expressing, Activation Assay, Injection, Western Blot